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Item Effect of combined nitrogen on growth and nodule function or Pueraria phaseoloides(Rubber Research Institute. Agalawatta, 1991) Jayasinghe, C.K.; Wettasinghe, D.S.Nodulation and nitrogen fixation rates of most of the legumes are reported to be severely affected when they are grown in soils high in available nitrogen. Furthermore, this phenomenon may become more critical when legumes are grown in multiple cropping systems like Pueraria phaseoloides in rubber plantatons where ono legume counterpart is fertilized with nitrogenous fertilizer. Results of experiments conducted in seeding agar to find the effect of nitrogen on growth and nodule function of P. phaseoloides showed that nodulation of P. phaphaeoloides was inhibited at a concentration between 24.4 and 44.8 ppm nitrogenItem Effect of incubation time on acetylene reduction activity of common cover crops of Sri Lankan rubber plantation(Rubber Research Institute. Agalawatta, 1989) Jayasinghe, C.K.; Parker, C.A.; Kulasooriya, S.A.A wide range of incubation time from a few minutes to severl hours have been used with defferent plant systems in investigations on acetylene reduction assays. However, the duration of incubation period is of utmost importance in obtaining nitrogen fixation estimates using this technology. Time course experiment showed that acwtylene reduction by nodules of P. phaseoloides and D. ovalifolium started without a lag but rate of the nitrogenasse activity was reduced 4 hours after incubation while M. invisa showed a considerable activity even at the 8th hour of incubation. In the second experiment which was conducted to observe the relationship between acetylene reduction activity and incubation time upto one our, there was a linear relationship for both P. phaseoloides (r = +0.9832) and D. ovalifolium (r = + 0.99961)Item Diurnal variation in nitrogenase activity of common cover crops in rubber plantations of Sri Lanka(Rubber Research Institute. Agalawatta, 1989) Jayasinghe, C.K.; Parker, C.A.; Kulasooriya, S.A.Diurnal fluctuations in nitrogenase activity (acetylene reducing activity) have been observed in several nitrogen fixing systems. All experimental plants viz. P. phasides D. ovalifolium and M. invisa demonstrated diuanal rhythm withlight intensity. The lowest acetylene reduction activiteswere detected around midnight and they were activelyu fixing nitrogen by 8.00 am. M. invisa responded to high soil temperature in addition to light intensity. A marked drop in acetylene reduction activity was observed by about 4.00pm when the soil temperature was around 38ºCItem Effect of combined nitrogen on nodule function of leguminous crops with special reference to tropical soils(Rubber Research Institute. Agalawatta, 1991) Jayasinghe, C.K.The effect of combined nitrogen on nitrogen fixation has been discussed by several investigators and from their findings it could be concluded that inorganic nitrogen may have either stimulatory, neutral of negative effect on nitrogen fixation depending on the type of legome and soil condition. It was reported in some instances that low levels of combined nitrogen especially when applied as a "Starter" fertilizer at germination, stimulates plant growth and enhances nodule mass and nitrogen fixation. On the other hand when most of the legumes are grown in soil high in available nitrogen, the nitrogen fixation rate is severely affected. This phenomenon may become more critical when legumes are grown as cover crops where non legume counterpart is fertilized with nitrogenous fertilizer. The degree of inhibition appears to vary with sevaral factors vis, concentration of combined nitrogen, host species, cultivar, strain of root nodule bacteria, rate of inculation, light intensity and temperature. However, the most critical factor is the concentration of combined nitrogen and it has been shown that very low concentrations such as 240 ppm N reducedItem Cross inoculation of Bradyrhizobia among three common legume cover crops in rubber plantations of Sri Lanka(Rubber Research Institute. Agalawatta, 1989) Jayasinghe, C.K.; Wettasinghe, S.Mixtures of legume cover crops are common in rubber plantations of Sri Lanka during the immature phase of rubber, and changes in ther composition occure with the changes in environment conditions due to the growth of canopies in rubber plants. Bradythizobial isolates from Pueraria phaseloides and desmodium ovalifolium were able to nodulate both legumes, but non of the isolates, including CB 756 (C.S.I.R.O collection, Queensland) produced nodules on Mimosa invisa.Item Seasonal variations in nodule activity of Pueraria phaseoloides (Roxb) Benth(Rubber Research Institute. Agalawatta, 1990) Jayasinghe, C.K.; Parker, C.A.; Kulasooriya, S.A.The age of Pueraria phaseoloides plants had a marked effect upon the rate of acetylene reduction in their nodules. Acetylene reduction activity increased with the age of the plant and maximum efficiency of nodule activity was detected 60 days after sowing, followed by a marked decline on the 120th day and continued at a low rate. Dry matter yield and total nitrogen in plants was more or less constant thrugh out the assay period.Item Native root-nodule bacterial populations in rubber growing soils and forest soils(Rubber Research Institute. Agalawatta, 1986) Jayasinghe, C.K.; Parker, C.A.; Kulasooriya, A.Item Effect of different levels of supplemental nitrogen on CO2 assimilation rates and productivity of Pueraria phaseoloides (Roxb) Benth and Desmodium ovalipolium (prain) wall. ex ridely grown in agalawatta series soil(Rubber Research Institute. Agalawatte, 1985) Nugawela, A.; Jayasinghe, C.K.; Aluthhewage, R.; Wettasinghe, S.Plant dry matter production is linked to CO2 assimilation rates, respiratory losses and total leaf area. Experiments were undertaken to determine the effect of different levels of supplimental nitrogen given to legume cover crops, on their CO2 assimilation rates and productivity. They were found to be highest for Pueraria phaseoloides and Desmodium ovaliforlium when given around 140 and 35 ppm of supplimental nitrogen. Reciprocal of specific leaf area and laef nitrogen content were also determined and they showed a positive linear relationship with supplemental nitrogen levels. How nitrogen could a influence the CO2 assimilation rates of cover crops studies are discussed.Item Nodulation nitrogenase activity and major nutrient constituents of common cover crop legumes during early growth(Rubber Research Institute. Agalawatta, 1989) Jayasinghe, C.K.; Parker, C.A.; Kulasooriya, S.A.